Epitope mapping in TSPAN7 a novel T1D related beta cell autoantigen

This abstract has open access
Abstract Summary

BACKGROUND: Tetraspanin 7 is an integral membrane protein that is expressed in the pancreatic beta cells. It is also an autoantigen target in type 1 diabetes. Autoantibodies to tetraspanin7 are found in 35% of patients at disease onset as compared to < 1% of healthy control subjects. Since tetraspanin 7 span both cytosolic and extracellular compartments, we asked where the major autoantibody epitopes of this beta cell protein were located. METHODS: We prepared constructs that contained separate domains of the protein, expressed these as Nluc tagged proteins and measured patient autoantibody binding to each using a Luciferase Immunoprecipitation Assay. Epitopes in tetraspanin7 positive sera were mapped down to two soluble domains of the protein, extracellular domain 2 and cytoplasmic domain 3 (C3). We then performed alanine scanning of the C3 cytoplasmic domain and revealed the importance of amino acids 239 and 243-247 for binding antibodies from patient sera. RESULTS: We define an autoantibody epitope within a cytoplasmic loop of tetraspanin 7, thus revealing a novel T1D relevant epitope.

Submission ID :
IDS7037
Submission Type
Abstract Topics
CRTD Center for Regenerative Therapies Dresden
CRTD Center for Regenerative Therapies Dresden
CRTD Center for Regenerative Therapies Dresden
Institute of Diabetes Research, Helmholtz Zentrum München, and Forschergruppe Diabetes, Klinikum rechts der Isar, TU München
CRTD Center for Regenerative Therapies Dresden
CRTD Center for Regenerative Therapies Dresden

Abstracts With Same Type

Submission ID
Submission Title
Submission Topic
Submission Type
Primary Author
4 visits

KEY DATES

Event dates:
Thursday 25 October - Monday 29 October 2018

Abstract submission deadline:
Monday 14 May 2018

Abstract notification:
July 2018

Early registration deadline:
Monday 3 September 2018

Registration deadline:
Monday 15 October 2018

Contact
British Society for Immunology
+44 (0)20 3019 5901
congress@immunology.org